Illumina reads from all 12 samples were pooled and compared to a set of 20 bivalvia mitochondrial genomes to identify reads with mitochondrial origin. This page was last edited on 9 december 2014, at 16. The native species ruditapes decussatus and the invasive species ruditapes philippinarum have an important ecological role and socioeconomic value, from the atlantic and mediterranean to the indopacific region. In this study, oxygen consumption oc, total ammonia excretion tam and clearance rate cr responses to the changes in seawater salinity 37, 30, 25 and 20. The production is in decline due to several factors such as perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam ruditapes.
In the present study, a multibiomarker approach was used to assess the biological effects of metal pollution in the southern lagoon of tunis, on clam ruditapes decussatus both in hot in summer and cold in winter seasons. This paper reports, for the first time, the complete mitochondrial genome of the grooved carpet shell, ruditapes decussatus, also known as the european clam. Metabolic responses of clams, ruditapes decussatus. Research article open access mrnaseq and microarray. All structured data from the file and property namespaces is available under the creative commons cc0 license. A total of 146 immunerelated genes were identified in the transcriptome of the mantle of r. Analysis of the 16s rrna gene allowed the identification of the strains as members of the genus marinomonas, sharing the highest similarity with marinomonas. Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam ruditapes decussatus larvae aquaculture international, aug 2016 amel medhioub, badreddine mechri, sondes bchir, younes limeyem, wissem slimani, mahjoub aouni. In 1948 it was estimated that populations of ruditapes decussatus and venerupis pullastra in the ria del burgo spain recovered in less than a year from a density of 15 to 3050 clamsm 2. Ruditapes decussatus, the manila clam ruditapes philippinarum, the pacific oyster crassostrea gigas and several species other clams, abalone, scallops can be tested the same way. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer.
Apr 14, 2015 the native species ruditapes decussatus and the invasive species ruditapes philippinarum have an important ecological role and socioeconomic value, from the atlantic and mediterranean to the indopacific region. Naturalization of the manila clam tapes philippinarum, an alien species, and establishment of a clam fishery within poole harbour, dorset volume 84 issue 5 a. International journal of systematic and evolutionary microbiology 61 10. Pescados mariscos moluscos pdf 579 1 linkedin slideshare. In the aquatic environment, they are subjected to the presence of different contaminants, such as mercury hg and its methylated form, methylmercury mehg. The harvesting of ruditapes decussatus mainly occurs in spain and france.
Thirty eight percent of the blast hits were obtained with members of the family veneridae followed by 27% with members of the family ostreidae see fig. Reproductive pattern of the clams, ruditapes decussatus and r. Clam stock on the western coast of cotentin english channel the manila clam ruditapes philippinarum adam and reeve 1850 was introduced into france in the 1970s and now dominates the european clam ruditapes decusattus linnaeus 1758 in the intertidal zone of the western cotentin english channel. Despite the large clam harvests, their repopulation is rapid. A biomonitor of trace elements pollution in sardinian coastal lagoons italy. Change in fatty acid composition and evaluation of lipids. If you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs. In spain, early records of mollusc fishing and consumption from the 16 th century are mainly about the flat oyster, and only rarely about clams, but they do mention the marketing of clams in portugal and other places.
A new threephase culture method for manila clam, ruditapes philippinarum, farming in northern china. Files are available under licenses specified on their description page. In this study, we characterized the transcriptome of male and female mature gonads in the european clam ruditapes decussatus, compared with that in the manila clam ruditapes philippinarum providing, for the first time in bivalves, information about transcription dynamics and sequence evolution of sexbiased genes. For this reason, interest in the immune genes in this species has recently increased. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various environmental stressors on. Glutathione stransferases gst, glutathione peroxidases gpx and metallothioneins mt are essential components of cellular detoxication systems. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer, from where it. May 12, 2011 the manila clam, ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. Intensive fishing for clams began in 1926 and 1927. Oxidative stress and damage biomarkers in clam ruditapes. Genoyster 20152018 validation of dnaparentage assignment panel for pacific oyster c. The grooved carpet shell clam ruditapes decussatus is the autochthonous european clam and the most appreciated from a gastronomic and economic point of view. A previous analysis on the cox1 gene of ruditapes decussatus reported a nucleotide diversity. Review history for the complete mitochondrial genome of.
Camino ordas and antonio figueras huerta download pdf 2 mb. The first known occurrence of a naturalized population of manila clam tapes philippinarum in uk waters is reported. In genes with pdistance 40%, the relationship is better explained by an exponential function red. Abalone mortalities in australia and clams mortalities in portugal, korea, china, japan. Despite the large number of animal complete mitochondrial genomes currently available in public databases, knowledge about mitochondrial genomics in invertebrates is uneven. Impacts of rearing techniques on growth, survival and. Review history for the complete mitochondrial genome of the. The isolates formed two aflp clusters and belonged to the genus vibrio, more precisely to the splendidus clade. Growth rate is one of the most important traits from the point of view of individual fitness and commercial production in mollusks, but its molecular and physiological basis is poorly known. Isolated from the clams ruditapes philippinarum and ruditapes decussatus. Download fulltext pdf download fulltext pdf morphometric analysis of european clam ruditapes decussatus in morocco article pdf available in aacl bioflux 125. Transcriptome sequencing and microarray development for the. Bearded horse mussel modiolus barbatus and noahs ark shell arca noae are a species of interest for the diversifying shellfish aquaculture on the southeastern coast of the adriatic. In this study, oxygen consumption oc, total ammonia excretion tam and clearance rate cr responses to the changes in seawater salinity 37, 30, 25 and 20 were.
The leading journal in its field, meps covers all aspects of marine ecology, fundamental and applied. The present work describes the construction of the first r. This study investigated the effects of 48h heavy metal exposure upon the metabolic profiles of ruditapes decussatus and ruditapes philippinarum using 1h nmr metabolomics. Journal of the marine biological association of the united kingdom. The manila clam, ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. Phylogenetic analysis based on 16s rrna gene sequences showed that these marine bacteria were closely related to the recently described species vibrio. The eastern oyster crassostrea virginica, the carpet shell clam ruditapes decussatus, the manila clam ruditapes philippinarum, the pacific oyster crassostrea gigas and several species. It is one of the main native bivalve species cultured in europe. The grooved carpetshell clam ruditapes decussatus is native to the northern atlantic and mediterranean sea and has a high commercial value. Pdf microbiological monitoring of ruditapes decussatus. The editorial office will send you the word version of the file and i append a pdf version of it here, for the record.
In this study, phylogenetic analyses based on sequences of. The manila clam ruditapes philippinarum is a cultured bivalve with worldwide commercial importance, and diseases cause high economic losses. Download fulltext pdf microbiological monitoring of ruditapes decussatus from ria formosa lagoon south of portugal article pdf available in journal of coastal conservation 173. Gene expression profile analysis of manila clam ruditapes. Pdf microbiological monitoring of ruditapes decussatus from. Phylogenetic analysis based on 16s rrna gene sequences showed that these marine bacteria were closely related to the recently described species vibrio comitans, vibrio rarus and vibrio inusitatus 99. Pdf behaviour and biochemical responses of the marine. Naturalization of the manila clam tapes philippinarum. Pdf the clam ruditapes decussatus is a suspensionfeeding bivalve mollusc widely distributed in european waters and in the mediterranean. Virus present in the connective tissues sometimes larvae and juvenile mortalities. A study of gonadal development in ruditapes decussatus l. Both species were exposed to increasing concentrations of lead nitrate 10, 40, 60 and 100. Change in fatty acid composition and evaluation of lipids and. Uncovering the immunological repertoire of the carpet shell.
It is distributed worldwide and due to its ecological and economic interest has been proposed as a bioindicator. Introduced ruditapes philippinarum have become naturalized in many areas of europe, competing with native r. Pdf metallothioneins in the clam ruditapes decussatus. Influence of salinity on physiological response of the. Uncovering the immunological repertoire of the carpet. This means that randomly sampled individuals of ruditapes decussatus used in this work showed a much lower mtdna variability than ruditapes philippinarum siblings. Clams were collected in august 2015 and february 2016 from three sites of the lagoon and from louza considered a reference site. Facultative anaerobic marine bacteria isolated from cultured clams ruditapes decussatus and ruditapes philippinarum were previously investigated using aflp and 16s rrna gene sequences analyses. Survivin immunoreactivity in the gastric mucosa of rats feedind with carpet shell clam ruditapes decussatus topics dardanelles, immunohistochemistry, stomach, carpet shell clam.
The complete mitochondrial genome of the grooved carpet. Burrowed in intertidal to sublittoral soft sediments, chiefly in sheltered areas. Pdf morphometric analysis of european clam ruditapes. Scope this procedure explains the diagnostic test used for the protistan perkinsus sp. The grooved carpet shell, or palourde clam, ruditapes decussatus, or venerupis decussatus, is a clam or bivalve mollusc in the family veneridae. It is distributed worldwide and due to its ecological and economic interest has been proposed as a bioindicator this species is one of the most popular and profitable mollusc of lagoonal and coastal sites in the mediterranean, where. Preliminary study of the clam ruditapes decussatus exposed in. We studied the expression of pigst, segpx, and mt transcripts in the digestive gland of dreissena polymorpha exposed to organic and metallic pollutants. The complete mitochondrial genome of the grooved carpet shell. Ruditapes decussatus is morphologically and ecologically similar. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader. Mussels from a control site were transplanted during 3, 15 and 30 days into. A linear function black dashed line describes the relationship between dnds and pdistance in genes with lower pdistance. Ruditapes philippinarum and ruditapes decussatus under hg.
Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam ruditapes decussatus larvae aquaculture international, aug 2016 amel medhioub, badreddine mechri, sondes bchir, younes limeyem, wissem slimani, mahjoub aouni, mohamed nejib medhioub. This is a straightforward analysis of several aspects of the mitochondrial genome sequence of this bivalve mollusk. The mitochondrial genome of ruditapes decussatus was not available in the databases, so we used the transcriptome data to generate a draft to be used as a guide for sanger sequencing. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various. The production is in decline due to several factors such as perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam ruditapes philippinarum. Jun 15, 2016 this study investigated the effects of 48h heavy metal exposure upon the metabolic profiles of ruditapes decussatus and ruditapes philippinarum using 1h nmr metabolomics.
This study focused on industrial effluent characterization and determination of its effects on the clam ruditapes decussatus. The main objective of the present study was to gain further insights into the immunological repertoire of r. In vitro culture of perkinsus atlanticus, a parasite of the carpet shell clam ruditapes decussatus by m. Download fulltext pdf download fulltext pdf behaviour and biochemical responses of the marine clam ruditapes decussatus exposed to phosphogypsum article pdf available in environmental. We have studied differential gene expression related to differences in growth rate in adult individuals of the commercial marine clam ruditapes decussatus. Naturalization of the manila clam tapes philippinarum, an. Seven nonmotile, facultatively anaerobic, alginolytic marine bacteria were isolated from the cultured clams ruditapes philippinarum and ruditapes decussatus. This page was last edited on 26 december 20, at 12. Introduced into poole harbour for aquaculture in 1988. Download fulltext pdf microbiological monitoring of ruditapes decussatus from ria formosa lagoon south of portugal article pdf available in. Parasite present in connective tissue and epithelial cells. Topics covered include microbiology, botany, zoology, ecosystem research, biological oceanography, ecological aspects of fisheries and aquaculture, pollution, environmental protection, conservation, and resource management.
In the aquatic environment, they are subjected to the presence of different contaminants, such as mercury hg and its methylated form. Comparative transcriptomics in two bivalve species offers. Survivin immunoreactivity in the gastric mucosa of rats feedind with carpet shell clam ruditapes decussatus topics dardanelles, immunohistochemistry, stomach, carpet shell clam, survivin. Comparative study of gonadal development of ruditapes. Metabolic responses of clams, ruditapes decussatus and. Preliminary study of the clam ruditapes decussatus exposed. Zipcodezoo this veneridaerelated article is a stub. The regional government of galicia spain has banned the use of manila clam seed for semiextensive use on beaches, and several authorities are. Nov 01, 2003 read preliminary study of the clam ruditapes decussatus exposed in situ to metal contamination and originating from the gulf of gabes, tunisia, bulletin of environmental contamination and toxicology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Icpoes analysis was further performed on the clams. The analysis results revealed that the effluent having acid ph and high concentrations of some chemicals, comparing to the norms, such as so42.
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